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Strain MS845

• CGSC#: 5771
• Strain Designation

  Designation	Source	Preference
  MS845	Soffer R.L.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 8 Mutations:

  Mutation	Location	Certainty	Display
  pro-3(del)	5.92	1	Δpro-3
  lac-85(del)	7.80	1	Δlac-85
  tsx-76	9.28	1	tsx-76
  aat-1	19.96	1	aat-1
  rpsL230(strR)	74.84	1	rpsL230(strR)
  malT1(LamR)	76.54	1	malT1(λR)
  xylA5	80.34	1	xylA5
  mtl-1	81.26	1	mtl-1

  
  
• Comments:
  • aat = locus for leucyl-, phenylalanyl-transfer RNA-protein transferase, which maps between 50-60 minutes.
• References:
  • Soffer, R.L., M. Savage 1974. A mutant of Escherichia coli defective in leucyl, phenylalanyl-tRNA-protein transferase. Proc.Natl.Acad.Sci.USA 71:1004-1007
  • Deutch, A.H., R.L. Soffer 1975. Regulation of proline catabolism by leucyl-phenylalanyl-tRNA-protein transferase. Proc.Natl.Acad.Sci.USA 72:405-408
  • Deutch, C.E., R.C. Scarpulla, E.B. Sonnenblick, R.L. Soffer 1977. Pleiotropic phenotype of an Escherichia coli mutant lacking leucyl-, phenylalanyl-transfer ribonucleic acid-protein transferase. J.Bacteriol. 129:544-546
  
  

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