E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain BW21116/pAH33

• CGSC#: 8165
• Strain Designation

  Designation	Source	Preference
  BW21116/pAH33	Wanner B.L.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 7 Mutations:

  Mutation	Location	Certainty	Display
  DE(argF-lac)169	6.21	1	Δ(argF-lac)169
  uidA3(del)::pir+	36.48	1	ΔuidA3::pir+
  rpoS396(Am)	61.75	0	rpoS396(Am)
  rph-1	82.20	1	rph-1
  hsdR514	98.74	1	hsdR514
  rob-1	99.85	1	rob-1
  creC510	99.89	1	creC510

  
  
• Comments:
• Episome/Plasmid: pAH33

  Plasmid Markers/Mutations:
  oriR6Kgamma
  bla(ApR)
  tetA
  tetR
  PhiaraBp-'araD'

• References:
  • Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.
  • Haldimann, A., L.L. Daniels, B.L. Wanner 1998. Use of new methods for construction of tightly regulated arabinose and rhamnose promoter fusions in studies of the Escherichia coli phosphate regulon. J.Bacteriol. 180:1277-1286
  
  

  Printable Strain Report