E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain CAG5050

• CGSC#: 8106
• Strain Designation

  Designation	Source	Preference
  CAG5050	Gross C.A.	1
  CSH135	Miller J.H.	5

  
  
• Sex(Hfr,F+,F-,or F'): F'
• 7 Mutations:

  Mutation	Location	Certainty	Display
  DE(pro-lac)	5.86	1	Δ(pro-lac)
  his-	44.00	1	his-
  gyrA-0(NalR)	50.00	1	gyrA-0(NalR)
  tyrA-	59.00	1	tyrA-
  rpsL-(strR)	74.84	1	rpsL-(strR)
  Mu dX(ApR,lac,CmR)	1000.00	1	Mu dX(ApR,lac,CmR)
  met-	1000.00	1	met-

  
  
• Comments:
  • This is a derivative of a strain for which allele numbers were not published and we've not been able to trace the derivation of that strain. Similarly, the F prime is probably a derivative of F128 but this has not been verified.
• Episome/Plasmid: F128(?)-(BU8305)

  Plasmid Markers/Mutations:
  lacZ8305::Mu cts62

• References:
  • Baker, T.A., M. Howe, C.A. Gross 1983. Mu dX, a derivative of Mu d1(lac Ap^r) which makes stable lacZ fusions at high temperature. J.Bacteriol. 156:970-974
  
  

  To request this strain visit the ECGRC website.

  
  

  Printable Strain Report