E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain DF1671

• CGSC#: 4889
• Strain Designation

  Designation	Source	Preference
  DF1671	Fraenkel D.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 4 Mutations:

  Mutation	Location	Certainty	Display
  eda-1	41.61	1	eda-1
  hisG1	45.01	1	hisG1
  rpsL115(strR)	74.84	1	rpsL115(strR)
  pgi-2	91.21	1	pgi-2

  
  
• Comments:
  • 1. This strain can be grown in a minimal medium containing malate (25mM), glycerol (12.5 mM) and casamino acids (0.1%).It should not be grown in the presence of glucose, which selects revertants.
  • 2.Reference: Hillman and Fraenkel 1975 J.Bacteriol.122:1175
  • The strain must be monitored closely for revertants of eda-1 or pgi-2.
• References:
  • Fradkin, J.E., D. Fraenkel 1971. 2-keto-3-deoxygluconate 6-phosphate aldolase mutants of Escherichia coli. J.Bacteriol. 108:1277-1283
  
  

  To request this strain visit the ECGRC website.

  
  

  Printable Strain Report