E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain ND40

• CGSC#: 5215
• Strain Designation

  Designation	Source	Preference
  ND40	Ohtsubo E.	1

  
  
• Sex(Hfr,F+,F-,or F'): F'
• 7 Mutations:

  Mutation	Location	Certainty	Display
  lacZ39(del)	7.81	1	ΔlacZ39
  LAM-	17.40	1	λ-
  trpA49(Am)	28.33	1	trpA49(Am)
  recA11	60.80	1	recA11
  relA1	62.71	0	relA1
  rpsL150(strR)	74.84	1	rpsL150(strR)
  spoT1	82.34	0	spoT1

  
  
• Comments:
  • Note the suprressor mutation on F100-13. This may be supE44. DE lacZ39 is a deletion of ~ 1/3 of lacZ. It has also been called lac4680.
• Episome/Plasmid: F100-13

  Plasmid Markers/Mutations:
  glnV0(AS)

• References:
  • Ohtsubo, E, MT Hsu 1978. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB. J. Bacteriol. 134(3):778-94.
  
  

  Printable Strain Report