E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain BW5328/pAH122

• CGSC#: 7860
• Strain Designation

  Designation	Source	Preference
  BW5328/pAH122	Wanner B.L.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 7 Mutations:

  Mutation	Location	Certainty	Display
  DE(argF-lac)169	6.21	1	Δ(argF-lac)169
  recA1	60.80	1	recA1
  rpoS396(Am)	61.75	0	rpoS396(Am)
  rph-1	82.20	1	rph-1
  hsdR514	98.74	1	hsdR514
  rob-1	99.85	1	rob-1
  creC510	99.89	1	creC510

  
  
• Comments:
  • phage P21 excision and integrase plasmid
• Episome/Plasmid: pAH122

  Plasmid Markers/Mutations:
  LAMcI857(ts)
  repA101(ts)
  oriR101
  Lambda pR-xis
  bla(ApR)
  int P21
  bla
  int$_{P21}$
  xis$_{P21}$([]).

• References:
  • Haldimann, A, BL Wanner 2001. Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria. J. Bacteriol. 183(21):6384-93.
  
  

  Printable Strain Report