E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Strain MLK53

• CGSC#: 7700
• Strain Designation

  Designation	Source	Preference
  MLK53	Georgopoulos C.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 4 Mutations:

  Mutation	Location	Certainty	Display
  LAM-	17.40	1	λ-
  lpxL10(ts)::mini-Tn10	24.03	1	lpxL10(ts)::mini-Tn10
  IN(rrnD-rrnE)1	73.74	1	IN(rrnD-rrnE)1
  rph-1	82.20	1	rph-1

  
  
• Comments:
• References:
  • Karow, M., O. Fayet, A. Cegielska, T. Ziegelhoffer, C. Georgopoulos 1991. Isolation and characterization of the Escherichia coli htrB gene, whose product is essential for bacterial viability above 33 degrees C in rich media. J.Bacteriol. 173:741-750
  • Karow, M, S Raina, C Georgopoulos, O Fayet 1991. Complex phenotypes of null mutations in the htr genes, whose products are essential for Escherichia coli growth at elevated temperatures Res. Microbiol. 142(2-3):289-294.
  • Karow, M., C. Georgopoulos 1991. Sequencing, mutational analysis, and transcriptional regulation of the Escherichia coli htrB gene. Mol.Microbiol. 5:2285-2292
  
  

  Printable Strain Report