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CGSC, The Coli Genetic Stock Center

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Strain ts9

• CGSC#: 5593
• Strain Designation

  Designation	Source	Preference
  ts9	Flaks J.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 16 Mutations:

  Mutation	Location	Certainty	Display
  leuB6(Am)	1.74	1	leuB6(Am)
  fhuA2::IS2	3.61	1	fhuA2::IS2
  lacY1	7.79	1	lacY1
  tsx-1	9.28	1	tsx-1
  glnX44(AS)	14.99	1	glnX44(AS)
  gal-6	16.96	1	gal-6
  LAM-	17.40	1	λ-
  hisG1	45.01	1	hisG1
  argG6	71.48	1	argG6
  malT1(LamR)	76.54	1	malT1(λR)
  xyl-7	80.34	1	xyl-7
  mtlA2	81.26	1	mtlA2
  metB1	88.94	1	metB1
  coaA1(ts)	89.92	1	coaA1(ts)
  rplL9(L?)	90.06	1	rplL9(L?)
  ilu-1(ts)	1000.00	1	ilu-1(ts)

  
  
• Comments:
  • The mutation coaA1, originally called rts-1, confers temperature-sensitivity on this strain. The mutation ilu-1 confers a temperature-sensitive requirement for isoleucine.
• References:
  • Flaks, J., P.S. Leboy, E.A. Birge, C.G. Kurland 1966. Mutations and genetics concerned with the ribosomes. Cold Spr.Harb.Symp.Quant.Biol. 31:623-631
  • Watson, RJ, J Parker, NP Fiil, JG Flaks, JD Friesen 1975. New chromosomal location for structural genes of ribosomal proteins. Proc. Natl. Acad. Sci. U.S.A. 72(7):2765-9.
  • Song, W.J., S. Jackowski 1992. Cloning, sequencing, and expression of the pantothenate kinase (coaA) gene of Escherichia coli. J.Bacteriol. 174:6411-6417
  • Song, W.-J., S. Jackowski 1992. coaA and rts are allelic and located at kilobase 3532 on the Escherichia coli physical map. J.Bacteriol. 174:1705-1706
  • Siri, M., S.K. Westaway, S.J. Hayflick 2004. Pers.Commun. 6-:2004
  
  

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