E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain DS121

• CGSC#: 7565
• Strain Designation

  Designation	Source	Preference
  DS121	Rahuel-Clermont S.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 4 Mutations:

  Mutation	Location	Certainty	Display
  LAM-	17.40	1	λ-
  epd-11(del)::Ery	66.19	1	Δepd-11::Ery
  IN(rrnD-rrnE)1	73.74	1	IN(rrnD-rrnE)1
  rph-1	82.20	1	rph-1

  
  
• Comments:
  • These gap/epd-deleted strains should be grown on minimal supplemented with casamino acids, Trp, Met, Thi, succinate and glycerol plus antibiotic. They will not grow on LB or glucose media.
• References:
  • Seta, F.D., S. Boschi-Muller, M.L. Vignais, G. Branlant 1997. Characterization of Escherichia coli strains with gapA and gapB genes deleted. J.Bacteriol. 179:5218-5221
  
  

  To request this strain visit the ECGRC website.

  
  

  Printable Strain Report