Strain CD4
- CGSC#: 5096
- Strain Designation
Designation Source Preference CD4 Epstein W. 1 - Sex(Hfr,F+,F-,or F'): Hfr
- Hfr Point of Origin#: 2A
- PO Map Position: 12.20
- Direction of Marker Transfer: Counterclockwise
- 8 Mutations:
Mutation Location Certainty Display metD-88 4.74 1 metD-88 pro-3(del) 5.92 1 Δpro-3 DE(cod-lacI)6 7.60 1 Δ(cod-lacI)6 tsx-76 9.28 1 tsx-76 LAM- 17.40 1 λ- relA1 62.71 1 relA1 malT36(LamR) 76.54 1 malT36(λR) metB1 88.94 1 metB1 - Comments:
- Both donor and recipient must be met-(preferably same mutation) in order to use this marker. Low concentration of D-methionine (5-10ug/ml) works better in selection.
- References:
Printable Strain Report