E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain ND19

• CGSC#: 5205
• Strain Designation

  Designation	Source	Preference
  ND19	Ohtsubo & Davidson	1

  
  
• Sex(Hfr,F+,F-,or F'): F'
• 5 Mutations:

  Mutation	Location	Certainty	Display
  galT23	17.01	1	galT23
  LAM-	17.40	1	λ-
  trp-56	28.00	1	trp-56
  recA41	60.80	1	recA41
  rpsL178(strR)	74.84	1	rpsL178(strR)

  
  
• Comments:
  • 1.Ohtsubo lists this strain as being arg^-. Ogawa, who made the host strain N23-53, describes it as being Thr^-, Phe^-, Tyr^-. In our hands the strain requires aromatic amino
  • 2.acids and threonine.
• Episome/Plasmid: F153

  Plasmid Markers/Mutations:

• References:
  • Ohtsubo, E, MT Hsu 1978. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB. J. Bacteriol. 134(3):778-94.
  
  

  Printable Strain Report