E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain JC590

• CGSC#: 4499
• Strain Designation

  Designation	Source	Preference
  JC590	Clark A.J.	1

  
  
• Sex(Hfr,F+,F-,or F'): F'
• 14 Mutations:

  Mutation	Location	Certainty	Display
  leuB6(Am)	1.74	1	leuB6(Am)
  fhuA2::IS2	3.61	1	fhuA2::IS2
  lacY1	7.79	1	lacY1
  tsx-1	9.28	1	tsx-1
  glnX44(AS)	14.99	1	glnX44(AS)
  gal-6	16.96	1	gal-6
  LAM-	17.40	1	λ-
  hisG1	45.01	1	hisG1
  argG6	71.48	1	argG6
  rpsL104	74.84	1	rpsL104
  malT1(LamR)	76.54	1	malT1(λR)
  xyl-7	80.34	1	xyl-7
  mtlA2	81.26	1	mtlA2
  metB1	88.94	1	metB1

  
  
• Comments:
  • This strain must be grown on medium containing leu, his and lacking arg, met, thi. This F-prime is very unstable.
• Episome/Plasmid: F20

  Plasmid Markers/Mutations:

• References:
  • Clark, A.J., W.K. Maas, B. Low 1969. Production of a merodiploid strain from a double male strain of E. coli K12. Mol.Gen.Genet. 105:1-15
  
  

  Printable Strain Report