Site endA
- Name: endA
- Type: Gene
- Synonyms
b2945 MG1655 locus tag - Mnemonic: endonuclease
- Left End Point: 66.57
- Right End Point: 66.59
- Products:
DNA-specific endonuclease I - Direction: >
- Priority: 1
- 11 Alleles of This Gene
endA1 endA101 endA2 endA3 endA4 endA5 endA6(del-ins)::kan endA7(del-ins)::Cm endA720(del)::kan endA8(del:Frt:Tc)::Tc endA9(del-ins)::FRT - External Database Links:
Host Site Page Links EcoGene.org EG11336 EcoliWiki endA - Comment:
- select for mut. by methyl green (or Giemsa) staining for undegraded DNA. Endo I respons. for extensive DNA brkdown. endA- mutants improve miniprep dsDNA.
- References:
- Wright, M. 1971. Mutants of Escherichia coli lacking endonuclease I, ribonuclease I or ribonuclease II. J.Bacteriol. 107:87-94
- Jekel, M., W. Wackernagel 1994. Location of the endA gene coding for endonuclease I on the physical map of the Escherichia coli K-12 chromosome. J.Bacteriol. 176:1550-1551