E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site pKD118

• Name: pKD118
• Type: Plasmid
  Plasmid Sites:

  oriR6Kgamma

  tL3LAM(Ter)

  bla(ApR)

  rgnB(Ter)

  kan

  Pcp13-araE'

  
  
• Priority: 0
• Comment:
  • template plasmid carrying araE promoter Pcp13
• References:
  • Khlebnikov, A., K.A. Datsenko, T. Skaug, B.L. Wanner, J.D. Keasling 2001. Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter. Microbiol 147:3241-3247
  
  
• 1 Strains Carrying This Plasmid

  Name	Mutations	Genotype
  BW25141/pKD118	11	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ-, galU95, ΔuidA3::pir+, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD118