E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site pWM77

• Name: pWM77
• Type: Plasmid
  Plasmid Sites:

  oriR101

  bla(ApR)

  oriT RP4

  lacZ alpha

  ori f1,-

  MCS(KS)

  
  
• Priority: 0
• Comment:
  • Same as pWM71 except f1(-) and order of MCS is (KS). From pBluescript II(KS-), Stratagene.
• References:
  • Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.
  
  
• 1 Strains Carrying This Plasmid

  Name	Mutations	Genotype
  BW19094/pWM77	4	F-, Δ(codB-lacI)3, ΔphoA532, ΔuidA3::pir+, Δ(phnP-phnD)3330( phnC?), pWM77