E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site pAH34

• Name: pAH34
• Type: Plasmid
  Plasmid Sites:

  oriR6Kgamma

  MCS

  oriT RP4

  bla-1(ApR,dPstI)

  ori f1,+

  lacZ alpha

  
  
• Priority: 0
• Comment:
  • Differs from pWM71 by removal of PstI site in {\it bla} gene, so generally more useful than pWM71.
• References:
  • Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.
  
  
• 1 Strains Carrying This Plasmid

  Name	Mutations	Genotype
  BW21038/pAH34	7	F-, Δ(codB-lacI)3, ΔphoA532, ΔuidA3::pir+, recA1, rph-1, Δ(phnP-phnD)3330( phnC?), mini-Tn5Lac4(CamR,lacIQ), pAH34