E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site folA

• Name: folA
• Type: Gene
  Synonyms

  b0048	MG1655 locus tag
  tmrA

  
  
• Mnemonic: Folate
• Left End Point: 1.07
• Right End Point: 1.08
  Products:

  dihydrofolate reductase

  DHFR

  
  
• Direction: >
• Priority: 1
  3 Alleles of This Gene

  folA201(del)::kan

  folA207(del)::kan

  folA791-YFP(::cat)

  
  
• External Database Links:

  Host Site	Page Links
  EcoGene.org	EG10326
  EcoliWiki	folA

  
  
• Comment:
  • trimethoprim resistance. Selectable phenotype. Increase, change: trimethoprim resistance.
• References:
  • Sheldon, R., S. Brenner 1976. Regulatory mutants of dihydrofolate reductase in Escherichia coli K12. Mol.Gen.Genet. 147:91-97
  
  
  • Stone, D., A.W. Phillips, J.J. Burchall 1977. The amino-acid sequence of the dihydrofolate reductase of a trimethoprim-resistant strain of Escherichia coli. Eur.J.Biochem. 72:613-624
  
  
  • Smith, D.R., J.M. Calvo 1979. Regulation of dihydrofolate reductase synthesis in Escherichia coli. Mol.Gen.Genet. 175:31-38
  
  
  • Rood, J.I., A.J. Laird, J.W. Williams 1980. Cloning of the Escherichia coli K-12 dihydrofolate reductase gene following Mu-mediated transposition. Gene 8:255-265
  
  
  • Andresson, O.S., J.E. Davies 1980. Isolation and characterization of lambda transducing phages for the E. coli genes ksgA and pdxA. Mol.Gen.Genet. 179:201-209
  
  
  • Smith, D.R., J.M. Calvo 1982. Nucleotide sequence of dihydrofolate reductase genes from trimethoprim-resistant mutants of Escherichia coli. Evidence that dihydrofolate reductase interacts with another essential gene product. Mol.Gen.Genet. 187:72-78
  
  
  • Smith, D.R., J.M. Calvo 1982. Nucleotide sequence of the E. coli gene coding for dihydrofolate reductase. Nucleic Acids Res. 8:2255-2274
  
  
  • Iwakura, M., Y. Shimura, K. Tsuda 1982. Cloning of dihydrofolate reductase gene of Escherichia coli K12. J.Biochem.(Tokyo) 91:1205-1212
  
  
  • Pinn, P.A., K.J. Towner, F.W. O'Grady 1983. Genetic analysis of chromosomal resistance to trimethoprim derived from clinical isolates of Escherichia coli. J.Gen.Microbiol. 128:85-92
  
  
  • Blanchin-Roland, S., S. Blanquet, J.M. Schmitter, G. Fayet 1986. The gene for Escherichia coli diadenosine tetraphosphatase is located immediately clockwise to folA and forms an operon with ksgA. Mol.Gen.Genet. 205:515-522