E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site pWM91

• Name: pWM91
• Type: Plasmid
  Plasmid Sites:

  oriR6Kgamma

  bla(ApR)

  oriT RP4

  ori f1,+

  lacZ alpha

  [sacB]$_{subtilis}$

  MCS(SK)

  
  
• Priority: 0
• Comment:
  • F1 ori in (+) orientation and lacZ from pBluescript II(SK+); unique polylinker sites ApaI, BamHI, NotI, SacI, SmaI, SpeI, XhoI.
• References:
  • Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.
  
  
• 1 Strains Carrying This Plasmid

  Name	Mutations	Genotype
  BW19335/pWM91	4	F', Δ(codB-lacI)3, ΔphoA532, ΔuidA3::pir+, Δ(phnP-phnD)3330( phnC?), pWM91, F128-Tn10-11