E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site pCP20

• Name: pCP20
• Type: Plasmid
  SubSites:

  FLP

  Cm

  Ap

  
  
  Plasmid Sites:

  [cI857](lambda)(ts)

  bla(ApR)

  cat

  FLP([])

  repA101(ts)

  
  
• Priority: 0
• External Database Links:

  Host Site	Page Links
  CGSC	pCP20_Seq.txt
  CGSC	pCP20_Map.pdf

  
  
• Comment:
  • pCP20 has the yeast Flp recombinase gene, FLP, chloramphenicol and ampicillin resistant genes, and temperature sensitive replication.
• References:
  • Cherepanov, PP, W Wackernagel 1995. Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene 158(1):9-14.
  
  
• 2 Strains Carrying This Plasmid

  Name	Mutations	Genotype
  BL21(DE3)/pCP20	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal+]K-12(λS), hsdS10, pCP20
  BT340	11	F-, Δ(argF-lac)169, φ80dlacZ58(M15), glnX44(AS), λ-, rfbC1, gyrA96(NalR), recA1, endA1, spoT1, thiE1, hsdR17, pCP20