E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site ligA

• Name: ligA
• Type: Gene
  Synonyms

  b2411	MG1655 locus tag
  dnaL
  lig
  pdeC

  
  
• Mnemonic: Ligase
• Left End Point: 54.45
• Right End Point: 54.50
  Products:

  DNA ligase

  
  
• Direction: <
• Priority: 1
  4 Alleles of This Gene

  ligA251(ts)

  ligA321

  ligA4

  ligA791-YFP(::cat)

  
  
• External Database Links:

  Host Site	Page Links
  NCBI/GenBank	M24278
  EcoGene.org	EG10534
  EcoliWiki	ligA

  
  
• Comment:
  • Selectable phenotype. Increase colonies "nibbled" by phage T4 ligase- mutants. Substantial loss, in increased strain: resistance to phage T4 ligase- mutants. See also RNA ligase ligR
• References:
  • Gellert, M., M.L. Bullock 1970. DNA ligase mutant of Escherichia coli. Proc.Natl.Acad.Sci.USA 67:1580-1587
  
  
  • Ishino, Y., H. Shinagawa, K. Makino, S. Tsunasawa, F. Sakiyama, A. Nakata 1986. Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli. Mol.Gen.Genet. 204:1-7
  
  
  • Ivanchenko, M., K. van Holde, J. Zlatanova 1996. Prokaryotic DNA ligases unwind superhelical DNA. Biochem.Biophys.Res.Commun. 226:498-505