E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site melR

• Name: melR
• Type: Gene
  Synonyms

  b4118	MG1655 locus tag

  
  
• Mnemonic: Melibiose
• Left End Point: 93.51
• Right End Point: 93.53
• Direction: <
• Priority: 1
  2 Alleles of This Gene

  melR744(del)::kan

  melR791-YFP(::cat)

  
  
• External Database Links:

  Host Site	Page Links
  EcoGene.org	EG11230
  EcoliWiki	melR

  
  
• Comment:
  • Selectable phenotype. Regulatory, in lacI: growth on raffinose. Regulatory, loss of catabolite repression: growth on melibiose + a-methylglucoside.
• References:
  • Lester, B., D.M. Bonner 1957. Genetic control of raffinose utilization in Escherichia coli. J.Bacteriol. 73:544-552
  
  
  • Webster, C., K. Kempsell, I.R. Booth, S.J. Busby 1987. Organization of the regulatory region of the Escherichia coli melibiose operon. Gene 59:253-263
  
  
  • Caswell, R., J. Williams, A. Lyddiatt, S. Busby 1992. Overexpression, purification and characterization of the Escherichia coli MelR transcription activator protein. Biochem.J. 2):493-499
  
  
  • Bourgerie, S.J., C.M. Michan, M.S. Thomas, S.J. Busby, E.I. Hyde 1997. DNA binding and DNA bending by the MelR transcription activator protein from Escherichia coli. Nucleic Acids Res. 25:1685-1693