E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site pKK3535

• Name: pKK3535
• Type: Plasmid
  SubSites:

  rrsB

  gltT

  rrlB

  rrfB

  
  
  Plasmid Sites:

  bla(ApR)

  ori pMB1

  
  
• Priority: 0
• Comment:
  • This plasmid contains the rrnB cluster cloned into the BamHI site of pBR322
• References:
  • Brosius, J., A. Ullrich, M.A. Raker, A. Gray, T.J. Dull, R.R. Gutell, H.F. Noller 1981. Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RLNA operon of E. coli. Plasmid 6:112-118
  
  
• 2 Strains Carrying This Plasmid

  Name	Mutations	Genotype
  SQ141	8	F-, Δ(rrsH-aspU)794(::FRT), λ-, Δ(rrfG-rrsG)791(::FRT), Δ(rrfF-rrsD)793(::FRT), rph-1, Δ(rrsA-rrfA)792(::FRT), Δ(rrsB-rrfB)790(::FRT), Δ(rrsE-rrfE)789(::FRT), ptRNA67, pKK3535
  SQ171	9	F-, Δ(rrsH-aspU)794(::FRT), λ-, Δ(rrfG-rrsG)791(::FRT), Δ(rrfF-rrsD)793(::FRT), rph-1, Δ(rrsC-trpT)795(::FRT), Δ(rrsA-rrfA)792(::FRT), Δ(rrsB-rrfB)790(::FRT), Δ(rrsE-rrfE)789(::FRT), ptRNA67, pKK3535