E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site pTOF25

• Name: pTOF25
• Type: Plasmid
  Plasmid Sites:

  repA101(ts)

  kan

  cat

  sacB

  
  
• Priority: 0
• Comment:
  • Construction replacement vector derived from pKO3 and pUC4K. Cloning into pTOF25 using salI-smaI results in screenable loss of kan marker.
• References:
  • Merlin, C., S. McAteer, M. Masters 2002. Tools for characterization of Escherichia coli genes of unknown function. J.Bacteriol. 184:4573-4581
  
  
• 1 Strains Carrying This Plasmid

  Name	Mutations	Genotype
  EDCM71	11	F-, Δ(argF-lac)169, φ80dlacZ58(M15), glnX44(AS), λ-, rfbC1, gyrA96(NalR), recA1, endA1, spoT1, thiE1, hsdR17, pTOF25