E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation thyA710

• Name: thyA710
• Type: Unknown
• Mutation of Gene: thyA
• Approx. Map Location: 63.86
• Comments
  • Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• References
• 8 Strains Carrying This Mutation

  Name	Mutations	Genotype
  JE1011	15	F-, thr-1, araC14, leuB6(Am), secA216?, fhuA61?, lacY1, galT23, λ-, trp-84, his-215, thyA710, rpsL264(strR), xylA5, mtl-1, thiE1
  NS1	16	F-, thr-1, araC14, leuB6(Am), secA206(aziR), fhuA61, lpcA1, lacY1, galT23, λ-, trp-84, his-215, thyA710, rpsL263(strR), xylA5, mtl-1, thiE1
  NS2	16	F-, thr-1, araC14, leuB6(Am), secA206(aziR), fhuA16, lpcA2, lacY1, galT23, λ-, trp-84, his-215, thyA710, rpsL263(strR), xylA5, mtl-1, thiE1
  NS3	16	F-, thr-1, araC14, leuB6(Am), secA206(aziR), fhuA61, lacY1, galT23, λ-, trp-84, his-215, thyA710, rpsL263(strR), xylA5, mtl-1, thiE1, tfr-3
  NS4	16	F-, thr-1, araC14, leuB6(Am), secA206(aziR), fhuA61, lacY1, galT23, λ-, trp-84, his-215, thyA710, lpcB4, rpsL263(strR), xylA5, mtl-1, thiE1
  ST222	16	F-, thr-1, araC14, leuB6(Am), murC3(ts), secA216, fhuA61, lacY1, galT23, λ-, trp-84, his-215, thyA710, rpsL263(strR), xylA5, mtl-1, thiE1
  ST5	16	F-, thr-1, araC14, leuB6(Am), secA216, fhuA61, lacY1, galT23, λ-, trp-84, his-215, thyA710, rpsL263(strR), xylA5, mtl-1, murB101(ts), thiE1
  ST640	18	F-, thr-1, araC14, leuB6(Am), ddlB1(ts), secA216, fhuA61, lacY1, galT23, λ-, trp-84, his-215, rfbC1?, mgl-51?, thyA710, rpsL263(strR), xylA5, mtl-1, thiE1