E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation rbsD296::Tn10

• Name: rbsD296::Tn10
• Type: Tn
• Mutation of Gene: rbsD
• Approx. Map Location: 84.73
• Synonyms

  Designation	Where Used
  zie-296::Tn10

  
  
• Properties

  Property	Symbol
  tetracycline resistant

  
  
• Comments
  • cotransduces 68% with ilv, counterclockwise. Shown by PCR analysis to be within the rbsD gene at nucleotide 3,931,475 (84.744 min.)
• References
  • Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.
• 2 Strains Carrying This Mutation

  Name	Mutations	Genotype
  CAG18501	3	F-, λ-, rph-1, rbsD296::Tn10
  SK2210	11	F-, Δ(lacY-lacZ)286, tsx-3?, glnX44(AS)?, λ-, lacZ95=phi80dIIlacZ9, hisG4(Oc), rpsL281(strR), xyl-7, mtl-1, rbsD296::Tn10, ΔargH1