E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation zfb-223::Tn10

• Name: zfb-223::Tn10
• Type: Tn
• Approx. Map Location: 51.45
• Properties

  Property	Symbol
  tetracycline resistant

  
  
• Comments
  • : insertion shown by PCR by Nichols et al. 1998 to be in orf b2274 (yfbO) at nucleotide 2,387,022 (51.453 min.)
  • cotransduces 18% with purF. The order is zeb-223:: Tn10, purF, aroC
• References
  • Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.
• 3 Strains Carrying This Mutation

  Name	Mutations	Genotype
  CAG18484	3	F-, λ-, zfb-223::Tn10, rph-1
  BW18499	7	F-, Δ(codB-lacI)3, λ-, zfb-223::Tn10, ackA202, rpoS396(Am)?, rph-1, ΔglnL2001
  DC334	15	F-, lacY1 or lacZ4?, glnX44(AS), λ-, rfbC1, zfb-223::Tn10, aroC4, dsdA7, rpsL8 or rplL9(L?) or rpsL14, malT1(λR), xyl-7, mtlA2, ΔargH1, thiE1, fhuA2 or fhuA14, tsx-23 or tsx-25