E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation zfd-1::Tn10

• Name: zfd-1::Tn10
• Type: Tn
• Approx. Map Location: 53.70
• Properties

  Property	Symbol
  tetracycline resistant

  
  
• Comments
  • cotransduces 80% with dsdA, 35% with fadL and 20% with aroC. PCR analysis places the insertion between orf b2374 and orf b2375, at nucleotide 2,491,451 (53.7 min.)
• References
  • Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.
• 2 Strains Carrying This Mutation

  Name	Mutations	Genotype
  CAG18467	3	F-, λ-, zfd-1::Tn10, rph-1
  RS3116	13	F-, lacY1 or lacZ4, glnX44(AS), aroC4, fadL701(T2R), dsdA1, zfd-1::Tn10, rpsL8 or rplL9(L?) or rpsL14, malT1(λR), xyl-7, mtlA2, ilvD188(Oc), thiE1, tsx-23 or tsx-25