E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation pfkA203(del-ins)::FRT

• Name: pfkA203(del-ins)::FRT
• Type: Insertion
• Mutation of Gene: pfkA
• Approx. Map Location: 88.49
• Properties

  Property	Symbol
  deletion-insertion	del-ins

  
  
• Comments
• References
  • Lovingshimer, M.R., D.A. Siegele, G.D. Reinhart 2006. Construction of an inducible, pfkA and pfkB deficient strain of Escherichia coli for the expression and purification of phosphofructokinase from bacterial sources. Protein Expr.Purif. 46:475-482
• 2 Strains Carrying This Mutation

  Name	Mutations	Genotype
  RL254	11	F-, [araD139]B/r, lacIp-4000(lacIQ), e14-, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), rbsR22, pfkA203(del-ins)::FRT, Δ(fimB-fimE)632(::IS1), deoC1
  RL257	12	F-, [araD139]B/r, lacIp-4000(lacIQ), e14-, pfkB205(del-ins)::FRT, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), rbsR22, pfkA203(del-ins)::FRT, Δ(fimB-fimE)632(::IS1), deoC1