E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation thyA20

• Name: thyA20
• Type: Unknown
• Mutation of Gene: thyA
• Approx. Map Location: 63.86
• Comments
  • Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• References
• 3 Strains Carrying This Mutation

  Name	Mutations	Genotype
  AB2495	22	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), galK2(Oc), λ-, trp-35, Rac-0, hisG4(Oc), rfbC1, mgl-51, thyA20, rpsL31(strR), xylA5, mtl-1, argE3(Oc), thiE1, deo-72
  PK122	22	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, glnX44(AS), galK2(Oc), λ-, trp-35, Rac-0, hisG4(Oc), rfbC1, lysP37, thyA20, rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thiE1, deo-72
  KL252	23	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), galK2(Oc), λ-, trp-35, Rac-0, hisG4(Oc), rfbC1, mgl-51, thyA20, rpsE2111(SpcR), rpsL31(strR), xylA5, mtl-1, argE3(Oc), thiE1, deo-72