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Mutation lacZ608(Am)

• Name: lacZ608(Am)
• Type: Unknown
• Mutation of Gene: lacZ
• Approx. Map Location: 7.81
• Synonyms

  Designation	Where Used
  lacZ-	Zinder
  NG608

  
  
• Properties

  Property	Symbol
  amber(UAG) mutation	Am

  
  
• Comments
  • is an amber mutation (Newton et al. 1965 J. Mol. Biol. 14:290), aka lacZ^-NG608 by Zinder, in segment 10 of the lacZ gene (Zipser et al. 1970).
• References
  • Newton, W.A., J.R. Beckwith, D. Zipser, S. Brenner 1965. Nonsense mutants and polarity in the lac operon of Escherichia coli. J.Mol.Biol. 14:290-296
  • Zipser, D, S Zabell, J Rothman, T Grodzicker, M Wenk 1970. Fine structure of the gradient of polarity in the z gene of the lac operon of Escherichia coli. J. Mol. Biol. 49(1):251-4.
• 10 Strains Carrying This Mutation

  Name	Mutations	Genotype
  CY265	3	Hfr(PO2A), Δ(aroP-aceF)73, lacZ608(Am), λ-
  KL335	3	Hfr(PO2A), lacZ608(Am), lacI22, λ-
  KL333	4	Hfr(PO2A), leu-40?, Δ(aroP-aceF)73, lacZ608(Am), λ-
  S\O5052	4	F-, lacZ608(Am), purR96, rpsL999(strR), thi-
  YYC186	4	F-, Δ(aroP-aceF)73, zac-284::Tn10, lacZ608(Am), λ-
  YYC7	8	Hfr(PO2A), secA214(aziR)?, Δ(aroP-aceF)73, fhuA54?, lacZ608(Am), LAMcI857(ts), tonB54?, thiE1, poxA1
  YYC117	9	Hfr(PO2A), secA214(aziR)?, Δ(aroP-aceF)73, fhuA54?, lacZ608(Am), LAMcI857(ts), poxB11, tonB54?, rpsL104, thiE1
  KL324	11	F-, secA209(aziR), pro-48, lacZ608(Am), lacI22, λ-, trpA9605(Am), his-85(Am), gyrA19(NalR), rpsL171(strR), metE90, trpR55
  KL325	12	F-, secA209(aziR), pro-48, lacZ608(Am), lacI22, e14-, trpA9605(Am), his-85(Am), gyrA19(NalR), serV43(AS), rpsL171(strR), metE70, trpR55
  YYC188	12	Hfr(PO2A), secA214(aziR)?, Δ(aroP-aceF)73, fhuA54?, lacZ608(Am), LAMcI857(ts), zbj-927::Tn10, poxB11, pflB1, tonB54?, pps-4, rpsL104, thiE1