E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation cyaA854(del)

• Name: cyaA854(del)
• Type: Deletion
• Mutation of Gene: cyaA
• Approx. Map Location: 85.98
• Synonyms

  Designation	Where Used
  cya-854

  
  
• Properties

  Property	Symbol
  deletion	del

  
  
• Comments
  • is a 200 bp frameshift deletion that generates a new translation start (Glaser et al., below)
• References
  • Glaser, P, A Roy, A Danchin 1989. Molecular characterization of two cya mutations, cya-854 and cyaR1. J. Bacteriol. 171(9):5176-8.
  • Shah, S, A Peterkofsky 1991. Characterization and generation of Escherichia coli adenylate cyclase deletion mutants. J. Bacteriol. 173(10):3238-42.
• 6 Strains Carrying This Mutation

  Name	Mutations	Genotype
  LJ2809	4	F-, fruR11::Tn10, xyl-7, ΔcyaA854, ΔargH1
  LJ2805	5	F-, fruR11::Tn10, Δ(codB-lacI)3, xyl-7, ΔcyaA854, glp-8306
  CA8306	6	Hfr(PO1), λ-, e14-, relA1, spoT1, ΔcyaA854, thiE1
  CA8404	8	Hfr(PO1), λ-, e14-, relA1, rpsL136(strR), crp-1004, spoT1, ΔcyaA854, thiE1
  CA8439	8	Hfr(PO), λ-, e14-, relA1, rpsL136(strR), Δcrp-39, spoT1, ΔcyaA854, thiE1
  CA8445	8	Hfr(PO1), λ-, e14-, relA1, rpsL136(strR), Δcrp-45, spoT1, ΔcyaA854, thiE1