E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation ileS1

• Name: ileS1
• Type: Unknown
• Mutation of Gene: ileS
• Approx. Map Location: 0.48
• Comments
  • is an allele which results in a 300-fold higher apparent Km for Ile and can grow only when isoleucine is overproduced or added to the medium (and a 4-fold derepression of Ile synthesis enzymes).
• References
  • Iaccarino, M., P.E. Berg 1971. Isoleucine auxotrophy as a consequence of a mutationally altered isoleucyl-transfer ribonucleic acid synthetase. J.Bacteriol. 105:527-537
• 1 Strains Carrying This Mutation

  Name	Mutations	Genotype
  MI1	3	F-, ileS1, trpA36, ΔargH1