E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation zad-220::Tn10

• Name: zad-220::Tn10
• Type: Tn
• Approx. Map Location: 3.24
• Synonyms

  Designation	Where Used
  yadC

  
  
• Properties

  Property	Symbol
  tetracycline resistant

  
  
• Comments
  • cotransduces 96-98% with panD. The insertion is in orf yadC (at nucleotide 150,100, minute 3.235).
• References
  • Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.
• 4 Strains Carrying This Mutation

  Name	Mutations	Genotype
  CAG12025	3	F-, zad-220::Tn10, λ-?, rph-1
  SJ2	7	Hfr(PO1), panB6, zad-220::Tn10, λ-, e14-, relA1, spoT1, thiE1
  SJ16	8	F-, panD2, zad-220::Tn10, λ-, gyrA216(NalR), relA1, spoT1, metB1, λR
  DV1	9	F-, panD2, zad-220::Tn10, λ-, gyrA216(NalR), relA1, panF11, spoT1, metB1, λR?