Mutation rpoD285(ts)
- Name: rpoD285(ts)
- Type: Unknown
- Mutation of Gene: rpoD
- Approx. Map Location: 69.21
- Properties
Property Symbol Temperature Sensitive ts - Comments
- is a short deletion that makes an unstable σ70 protein and this instability leads to temperature sensitive growth.
- References
- Harris, J.D., J.S. Heilig, I.I. Martinez, R. Calendar, L.A. Isaksson 1978. Temperature sensitive Escherichia coli mutant producing a temperature-sensitive sigma subunit of DNA-dependent RNA polymerase. Proc.Natl.Acad.Sci.USA 75:6177-6181
- Hu, J.C., C.A. Gross 1983. Marker rescue with plasmids bearing deletions in rpoD identifies a dispensible part of E.coli sigma factor. Mol.Gen.Genet. 191:492-298
- Calendar, R., J.. Erickson, C. Halling, A. Nolte 1988. Deletion and insertion mutations in the rpoH gene of Escherichia coli that produce functional sigma-32. J.Bacteriol. 170:3479-3484
- Helmann, J.D., M.J. Chamberlin 1988. Structure and function of bacterial sigma factors. Annu.Rev.Biochem. 57:839-872
- 1 Strains Carrying This Mutation
Name Mutations Genotype UQ285 4 F-, lacZ4, λ-, rpoD285(ts), argG75