E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation Phi P_cp18 araE533

• Name: Phi P_cp18 araE533
• Type: Splice
• Approx. Map Location: 64.21
• Comments
• References
  • Khlebnikov, A., K.A. Datsenko, T. Skaug, B.L. Wanner, J.D. Keasling 2001. Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter. Microbiol 147:3241-3247
• 5 Strains Carrying This Mutation

  Name	Mutations	Genotype
  BW27271	8	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔaraEp-531::kan, φPcp18araE533, rph-1, Δ(rhaD-rhaB)568, hsdR514
  BW27784	8	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, Δ(araH-araF)570(::FRT), ΔaraEp-532::FRT, φPcp18araE533, Δ(rhaD-rhaB)568, hsdR514
  NCM533	8	F-, Δ(araD-araB)714, Δ(lacA-lacZ)880(::FRT), lacIp-4000(lacIQ), zah-2225::FRT, λ-, ΔaraEp-531::kan, φPcp18araE533, rph-1
  NCM534	8	F-, Δ(araD-araB)714, Δ(lacA-lacZ)880(::FRT), lacIp-4000(lacIQ), zah-2225::FRT, λ-, ΔaraEp-532::FRT, φPcp18araE533, rph-1
  BW27750	9	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, Δ(araH-araF)570(::FRT), ΔaraEp-531::kan, φPcp18araE533, rph-1, Δ(rhaD-rhaB)568, hsdR514