E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation thiC39::Tn10

• Name: thiC39::Tn10
• Type: Tn
• Mutation of Gene: thiC
• Approx. Map Location: 90.36
• Properties

  Property	Symbol
  tetracycline resistant

  
  
• Comments
  • From Kleckner strain NK5139. This thiinsertion shown more specifically by PCR to be within thiC, at nucleotide 4,192,143 (minute 90.363 -- Nichols et al. 1998.
• References
  • Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.
• 4 Strains Carrying This Mutation

  Name	Mutations	Genotype
  CAG18500	3	F-, λ-, rph-1, thiC39::Tn10
  NK5139	3	F-, λ-, IN(rrnD-rrnE)1, thiC39::Tn10
  CAG8160	4	Hfr(PO68), λ-, relA1, spoT1, thiC39::Tn10
  MPC529	22	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), galK2(Oc), λ-, Rac-0, hisG4(Oc), rfbC1, mgl-51, rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), gltT245(OS), thiE1, thiC39::Tn10