E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation DE(lacZ-lacI)875

• Name: DE(lacZ-lacI)875
• Type: Deletion
• Approx. Map Location: 7.79
• Comments
  • Transformation with pTAC3258, carrying an MluI cutback of the lac operon
• References
  • Rasmussen, L.J., P.L. Moller, T. Atlung 1991. Carbon metabolism regulates expression of the pfl (pyruvate formate-lyase gene in Escherichia coli. J.Bacteriol. 173:6390-6397
• 4 Strains Carrying This Mutation

  Name	Mutations	Genotype
  LJ24	9	F-, leuB6(Am), fhuA21, Δ(lacZ-lacI)875, lacY1, glnX44(AS), λ-, rfbC1, glpR200(glpc), thiE1
  ALO802	11	F-, leuB6(Am), fhuA21, lacY1, Δ(lacZ-lacI)875, aroL478::Tn10, glnX44(AS), λ-, rfbC1, glpR200(glpc), thiE1, hsdR0?
  ALO803	11	F-, leuB6(Am), fhuA21, lacY1, Δ(lacZ-lacI)875, glnX44(AS), λ-, rfbC1, aroK17::Cm, glpR200(glpc), thiE1, hsdR0?
  ALO807	12	F-, leuB6(Am), fhuA21, lacY1, Δ(lacZ-lacI)875, aroL478::Tn10, glnX44(AS), λ-, rfbC1, aroK17::Cm, glpR200(glpc), thiE1, hsdR0?