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Mutation lacZ118(Oc)

• Name: lacZ118(Oc)
• Type: Base Pair Substitution
• Mutation of Gene: lacZ
• Approx. Map Location: 7.81
• Synonyms

  Designation	Where Used
  lac U118

  
  
• Properties

  Property	Symbol
  ochre(UAA) mutation	Oc

  
  
• Comments
  • is the lacZ allele originating in Paris strain Hfr 3000 U118. It is an ochre mutation.
• References
  • Newton, W.A., J.R. Beckwith, D. Zipser, S. Brenner 1965. Nonsense mutants and polarity in the lac operon of Escherichia coli. J.Mol.Biol. 14:290-296
  • Epstein, W 1967. Transposition of the lac region of Escherichia coli. IV. Escape from repression in bacteriophage-carried lac genes. J. Mol. Biol. 30(3):529-43.
  • Norkins 1970. J.Mol.Biol. 51:663-0
  • Zabin, I, AV Fowler, JR Beckwith 1978. Position of the mutation in beta-galactosidase ochre mutant U118. J. Bacteriol. 133(1):437-8.
• 19 Strains Carrying This Mutation

  Name	Mutations	Genotype
  GM124	3	F-, lacZ118(Oc), rpsL275(strR), dam-4
  CAG18420	4	F-, lacZ118(Oc), lacI3098::Tn10kan, λ-, rph-1
  CAG18439	4	F-, lacZ118(Oc), lacI3042::Tn10, λ-, rph-1
  KL334	4	Hfr(PO2A), lacZ118(Oc), lacI22, λ-, lysA23
  KL316	5	F-, leu-40, lacZ118(Oc), pyr-42, rpsL143(strR), nal-16
  M7042	5	F-, lacZ118(Oc), λ-, relA1, rpsL150(strR), spoT1
  Hfr 3000 U118	6	Hfr(PO1), lacZ118(Oc), λ-, e14-, relA1, spoT1, thiE1
  TU6	10	F+, secA(aziR), lacZ118(Oc), λ-, lit-106, trpA9761(Am), IN(rrnD-rrnE)1, rph-1, ilvG865(Act), rho-6, trpE9829(Am) or trpE9997
  KL322	11	F-, secA209(aziR), pro-48, lacZ118(Oc), lacI22, trpA9605(Am), his-85(Am), gyrA19(NalR), recC22, rpsL171(strR), metE90, trpR55
  SS320	11	F-, secA209(aziR), pro-48, lacZ118(Oc), lacI22, λ-, trpA9705(Am), his-85(Am), gyrA19(NalR), rpsL171(strR), metE70, trpR55
  F597/EB54	12	F', secA209(aziR), pro-48, lacZ118(Oc), lacI22, λ-, trpA9605(Am), his-85(Am), gyrA19(NalR), recA1, rpsL171(strR), metE90, trpR55, F597
  KL321	12	F-, secA209(aziR), pro-48, lacZ118(Oc), lacI22, λ-, trpA9605(Am), his-85(Am), gyrA19(NalR), recB21, rpsL171(strR), metE90, trpR55
  KL323	12	F-, secA209(aziR), pro-48, lacZ118(Oc), lacI22, λ-, trpA9605(Am), his-85(Am), gyrA19(NalR), recA1, rpsL171(strR), metE70, trpR55
  KL390	12	F-, araC14, leuB6(Am), lacZ118(Oc), lacI22, λ-, serU132(AS), gyrA49(NalR), cysJ43, rpsL109(strR), xylA5, mtl-1, metE70
  SS3242	12	F-, secA219(aziR), pro-48, lacZ118(Oc), lacI22, λ-, trpA9605(Am), his-85(Am), gyrA19(NalR), rpsL171(strR), metE90, zjj-2002::Tn10, trpR55
  SS5210	12	F-, secA219(aziR), pro-48, lacZ118(Oc), lacI22, λ-, trpA9605(Am), his-85(Am), gyrA19(NalR), rpsL171(strR), metE90, zji-2001::Tn10, trpR55
  KL239	13	F-, secA209(aziR), pro-48, lacZ118(Oc), lacI22, λ-, e14-, trpA9605(Am), his-85(Am), gyrA19(NalR), serV43(AS), rpsL171(strR), metE90, trpR55
  KL384	14	F-, araC14, leuB6(Am), lacZ118(Oc), lacI22, λ-, serU132(AS), gyrA49(NalR), srl-1, alaS4(ts), argA21, rpsL109(strR), xylA5, mtl-1, metE70
  KL385	14	F-, araC14, leuB6(Am), lacZ118(Oc), lacI22, λ-, serU132(AS), gyrA49(NalR), srl-1, alaS5(ts), argA21, rpsL109(strR), xylA5, mtl-1, metE70