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Mutation recA441(ts)

• Name: recA441(ts)
• Type: Base Pair Substitution
• Mutation of Gene: recA
• Approx. Map Location: 60.80
• Synonyms

  Designation	Where Used
  tif-1	early designation, for thermal inducible filamentation

  
  
• Properties

  Property	Symbol
  Temperature Sensitive	ts

  
  
• Comments
  • formerly tif-1 (temp.-induced filamentation). Not a Rec^- phenotype, "constitutive protease activity" not requiring ss-DNA binding at hi temp.
• References
  • Templin, A., L.J. Margossian, A.J. Clark 1978. Suppressiblitiy of recA, recB and recC mutations by nonsense suppressors. J.Bacteriol. 134:590-596
  • Kawashima, H., T. Horii, T. Ogawa, H. Ogawa 1984. Organization of the recA gene of Escherichia coli. Mol.Gen.Genet. 193:288-292
  • Knight, KL, KH Aoki, EL Ujita, K McEntee 1984. Identification of the amino acid substitutions in two mutant forms of the recA protein from Escherichia coli: recA441 and recA629. J. Biol. Chem. 259(18):11279-83.
  • Lavery, P.E., S.C. Kowalczykowski 1990. Properties of recA442 protein-catalyzed DNA strand exchange can be attributed to an enhanced ability to compete with SSB protein. J.Biol.Chem. 265:4004-4010
• 9 Strains Carrying This Mutation

  Name	Mutations	Genotype
  JM888	5	Hfr(PO45), λ-, gyrA26(NalR), recA441(ts), relA1, thiE1
  C600-T44	10	F-, thr-1, leuB6(Am), fhuA21, lacY1, glnX44(AS), λ-, e14-?, recA441(ts), glpR200(glpc), thiE1
  DM1187	17	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, tsx-33?, glnX44(AS), galK2(Oc), λ-, sulA211, hisG4(Oc), recA441(ts), rpsL31(strR), xylA5, mtl-1, thiE1?, lexA51, lexA3(Ind-)
  KL788	17	F-, Δ(gpt-lac)5, tsx-35, λ-, sulA3, e14-, Rac-0?, rfbC1?, mgl-51?, recA441(ts), relA1?, rpsL31(strR), kdgK51?, mtl-1, spoT1?, thiE1?, lexA71::Tn5, creC510?
  KL790	17	F-, Δ(gpt-lac)5, tsx-35, λ-, sulA3, e14-, Rac-0?, rfbC1?, mgl-51?, recA441(ts), relA1?, rpsL31(strR), kdgK51?, xylA5, mtl-1, spoT1?, thiE1?, creC510?
  GW2707	18	F-, thr-1, araC14?, leuB6(Am), Δ(argF-lac)169, tsx-33, glnX44(AS)?, galK2(Oc), λ-, sulA211, hisG4(Oc), recA441(ts), rpsL31(strR), xylA5?, dinD1::Mud(Ap,lac)?, argE3(Oc), thiE1?, lexA71::Tn5, ilv(ts), pGW600
  DM1180	19	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, tsx-33?, glnX44(AS), galK2(Oc), λ-, sulA211, Rac-0, hisG4(Oc), rfbC1, mgl-51, recA441(ts), rpsL31(strR), kdgK51, xylA5, mtl-1, lexA3(Ind-)
  JM12	20	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS)?, galK2(Oc), λ-, Rac-0, hisG4(Oc), rfbC1, recA441(ts), rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thiE1
  KL789	20	F-, Δ(gpt-lac)5, tsx-35, λ-, ompF626?, zcb-222::Tn10, pyrD34 or pyrD36, sulA3, e14-, Rac-0?, rfbC1?, mgl-51?, recA441(ts), relA1?, rpsL31(strR), kdgK51?, xylA5, mtl-1, spoT1?, thiE1?, creC510?