E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation thyA3

• Name: thyA3
• Type: Unknown
• Mutation of Gene: thyA
• Approx. Map Location: 63.86
• Comments
  • Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• References
• 15 Strains Carrying This Mutation

  Name	Mutations	Genotype
  AB2299	9	F-, lacY1, glnX44(AS)?, gal-6?, λ-, hisG1, thyA3, argG6, xyl-7, ilvD132
  AB3950	9	F-, lacY1 or lacZ4, gal-6, hisG1, thyA3, argG6, xyl-7, ilvD132, thiE1?, spc-1
  AB2527	10	F-, lacY1, tsx-59, glnX44(AS), gal-6, λ-, hisG1, thyA3, argG6, xyl-7, ilvD132
  AB2531	10	F-, lacY1, tsx-59, glnX44(AS), gal-6, λ-, hisG1, thyA3, argG6, xyl-7, ilvD188(Oc)
  AB2533	10	F-, lacY1, tsx-59, glnX44(AS), gal-6, λ-?, purF1, thyA3, xyl-7, ilvD188(Oc), λR?
  AB2544	10	F-, lacY1, tsx-59, glnX44(AS), gal-6, LAMdgallysT102, hisG1, thyA3, argG6, xyl-7, ilvD188(Oc)
  AT2696	10	F-, glnX44(AS)?, λ-, hisG1, thyA3, lysA22, rpsL104, malT1(λR), xyl-7, mtlA2, metB1
  AT703	10	F-, lacY1, glnX44(AS)?, gal-6, manZ14, hisG1, purF59, thyA3, rpsL8 or rpsL104 or rpsL17, malT1(λR), pyrE41
  JR28	10	F-, lacY1 or lacZ4, tsx-64?, glnX44(AS)?, gal-6, hisG1, thyA3, metC69, rpsL8 or rplL9(L?) or rpsL17, malT1(λR), uxuA1
  AB2530	11	F-, lacY1, tsx-59, glnX44(AS), gal-6, λ-, hisG1, thyA3, argG6, xyl-7, metE46, deoC5?
  AT2699	11	F-, lacY1, tsx-1, glnX44(AS)?, gal-6, λ-, hisG1, thyA3, metC69, argG6, rpsL8 or rpsL104 or rpsL17, malT1(λR)
  EWH204	11	F-, lacY1, tsx-64, glnX44(AS)?, gal-6, recA1, thyA3, metK110(ts), argG6, rpsL8 or rpsL104 or rplL9(L?), metJ107, rpoB306
  EWH193	12	F-, lacY1, tsx-64, glnX44(AS)?, gal-6, hisG1, thyA3, metK110(ts), argG6, rpsL8 or rpsL104 or rplL9(L?), malT1(λR), metJ107, rpoB306
  JC500	18	F-, leuB6(Am), fhuA2::IS2, lacY1, glnX44(AS), gal-6, λ-, hns-1?, hisG1, rfbC1?, thyA3, galP63?, argG6, rpsL104, malT1(λR), xyl-7, mtlA2, metB1, deoC5
  JGC127	20	F-, leuB6(Am), fhuA2::IS2, lacY1, glnX44(AS), gal-6, λ-, cpxB11, hisG1, rfbC1?, thyA3, galP63?, argG6, rpsL104, malT1(λR), dnaM710(ts), zhh-922::Tn10, xyl-7, mtlA2, metB1, deoC5