E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation LAMpir

• Name: LAMpir
• Type: Insertion
• Approx. Map Location: 17.40
• Comments
  • Allows the replication of plasmids with an R6Kγ origin of replication
• References
  • McEachern, M.J., M. Filutowicz, D.R. Helinski 1985. Mutations in direct repeat sequences and in a conserved sequence adjacent to the repeats result in a defective replication origin in plasmid R6K. Proc.Natl.Acad.Sci.USA 82:1480-1484
• 4 Strains Carrying This Mutation

  Name	Mutations	Genotype
  S17-1 λpir/pLFT	8	F-, RP4-2(Km::Tn7,Tc::Mu-1), pro-82, LAMpir, recA1, endA1, thiE1, hsdR17, creC510, pLFT
  S17-1 λpir/pLFX	8	F-, RP4-2(Km::Tn7,Tc::Mu-1), pro-82, LAMpir, recA1, endA1, thiE1, hsdR17, creC510, pLFX
  S17-1 λpir/pUV5	8	F-, RP4-2(Km::Tn7,Tc::Mu-1), pro-82, LAMpir, recA1, endA1, thiE1, hsdR17, creC510, pUV5
  VPE42	13	F-, thr-1, leuB6(Am), zad-2084::Tn10, pcnB80, fhuA21?, cyn-101, lacY1, glnX44(AS), LAMpir, rfbC1, recA2111::RP4-2Tc::Mu, glpR200(glpc), thiE1