E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Mutation dcm-6

• Name: dcm-6
• Type: Unknown
• Mutation of Gene: dcm
• Approx. Map Location: 43.73
• Comments
  • has a Gln to Arg mutation,also a TGG to TGA termination mutation,which results in dcm-vsr message degradation and causes a Vsr^- phenotype (Dar 1993).
• References
  • Dar, M.E., A.S. Bhagwat 1993. Mechanism of expression of DNA repair gene vsr, an Escherichia coli gene that overlaps the DNA cytosine methylase gene, dcm. Mol.Microbiol. 9:823-833
• 13 Strains Carrying This Mutation

  Name	Mutations	Genotype
  GM1674	8	F', Δ(gpt-lac)5, glnX44(AS), galK2(Oc), galT22, λ-, dcm-6, dam-3, thiE1?, F128-20
  GM119	12	F-, fhuA2 or fhuA31, lacY1 or lacZ4, tsx-1 or tsx-78, glnX44(AS), galK2(Oc), galT22, λ-, dcm-6, dam-3, mtlA2, metB1, thiE1?
  GM272	12	F-, fhuA2 or fhuA31, lacY1 or lacZ4, tsx-1 or tsx-78, glnX44(AS), galK2(Oc), λ-, dcm-6, dam-3, mtlA2, metB1, thiE1?, hsdS21
  GM48	13	F-, thr-1, araC14, leuB6(Am), fhuA31, lacY1, tsx-78, glnX44(AS)?, galK2(Oc), galT22, λ-, dcm-6, dam-3, thiE1?
  JM110	14	F', thr-1, araC14, leuB6(Am), fhuA31, Δ(gpt-lac)0, tsx-78, glnX44(AS)?, galK2(Oc), galT22, λ-, dcm-6, rpsL199(strR), dam-3, thiE1?, F128-x
  ER2796	15	F-, fhuA2::IS2, glnX44(AS), λ-, e14-, trp-31, dcm-6, yedZ3069::Tn10, hisG1, argG6, rpsL104, Δdam-16::KanR, xyl-7, mtlA2, metB1, Δ(mcrC-mrr)114:IS10
  GM2151	15	F-, thr-1, araC14, leuB6(Am), fhuA31, lacY1, tsx-78, glnX44(AS), galK2(Oc), dcm-6, hisG1, rpsL136(strR), dam-13::Tn9, xylA5, mtl-1, thiE1
  ER3413	16	F-, fhuA2::IS2, glnX44(AS), λ-, e14-, trp-31, dcm-6, yedZ3069::Tn10, hisG1, argG6, yhdJ11, rpsL104, Δdam-16::KanR, xyl-7, mtlA2, metB1, Δ(mcrC-mrr)114:IS10
  GM2198	16	F-, thr-1, araC14, leuB6(Am), fhuA31, lacY1, tsx-78, glnX44(AS), galK2(Oc), λ-, dcm-6, hisG4(Oc), rpsL136(strR), dam-13::Tn9, xylA5, mtl-1, thiE1
  GM31	16	F-, thr-1, araC14, leuB6(Am), fhuA31, lacY1, tsx-78, glnX44(AS), galK2(Oc), galT22, λ-, dcm-6, hisG4(Oc), rpsL136(strR), xylA5, mtl-1, thiE1
  GM271	17	F-, araC14, leuB6(Am), fhuA31, lacY1, tsx-78, glnX44(AS), galK2(Oc), galT22, λ-, dcm-6, hisG4(Oc), rfbC1, rpsL136(strR), xylA5, mtl-1, thiE1, hsdR2
  GM2163	20	F-, araC14, leuB6(Am), fhuA13, lacY1, tsx-78, glnX44(AS), galK2(Oc), galT22, λ-, mcrA0, dcm-6, hisG4(Oc), rfbC1, rpsL136(strR), dam-13::Tn9, xylA5, mtl-1, thiE1, mcrB9999, hsdR2
  GM2929	21	F-, araC14, leuB6(Am), fhuA13, lacY1, tsx-78, glnX44(AS), galK2(Oc), galT22, λ-, mcrA0, dcm-6, hisG4(Oc), rfbC1, rpsL136(strR), dam-13::Tn9, xylA5, mtl-1, recF143, thiE1, mcrB9999, hsdR2