E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation glpD10

• Name: glpD10
• Type: Unknown
• Mutation of Gene: glpD
• Approx. Map Location: 76.73
• Comments
  • : Loss also results in stasis with gluconeogenic C sources, e.g., succinate. glpA or glpD mutants should be grown on min.salts+1% glycerol-free casein hydrolysate+1% glucose
  • : Loss of G-3-P dehydrogenase (aerobic) results in inability of strains to grow on glycerol or glycerol-3-P as sole carbon source.
• References
• 6 Strains Carrying This Mutation

  Name	Mutations	Genotype
  Lin 188	11	F-, fhuA2 or fhuA22, glnX44(AS)?, gal-6, λ-?, hisG1, rpsL8 or rplL9(L?) or rpsL17, malT1(λR), glpD10, mtlA2, thiE1, T2R?
  Lin 95	11	Hfr(PO2A), fhuA22, ΔphoA8, ompF627(T2R), fadL701(T2R), relA1, glpD10, pitA10, spoT1, rrnB-2, mcrB1, creC510
  Lin 204	12	Hfr(PO2A), fhuA22, ΔphoA8, ompF627(T2R), fadL701(T2R), relA1, glpD10, pitA10, spoT1, glpK30, rrnB-2, mcrB1, creC510
  Lin201	12	Hfr(PO2A), fhuA22, ΔphoA8, ompF627(T2R), glpA25, fadL701(T2R), relA1, glpD10, pitA10, spoT1, rrnB-2, mcrB1, creC510
  Lin 209	13	Hfr(PO2A), fhuA22, ΔphoA8, fadL701(T2R), relA1, glpR201, glpD10, pitA10, spoT1, glpK16, glpF17, rrnB-2, mcrB1, creC510
  Lin 118	14	F-, lacY1 or lacZ4, glnX44(AS), λ-, glpA24, purF1, fadL701(T2R), rpsL8 or rplL9(L?) or rpsL14, glpD10, pitA10, xyl-7, mtlA2, spoT1, ΔargH1, thiE1