E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation ampCp-1

• Name: ampCp-1
• Type: Unknown
• Mutation of Gene: ampCp
• Approx. Map Location: 93.80
• Synonyms

  Designation	Where Used
  ampA1

  
  
• Comments
  • is a promoter mutation; AmpC⁺ strains carrying this allele produce about 10X the amount of beta lactamase found in wild-type strains. In absence of other amp alleles, use 10-15 ug/ml Amp.
• References
  • Jaurin, B., T. Grundstrom, T. Edlund, S. Normark 1981. The E. coli beta-lactamase attenuator mediates growth rate-dependent regulation. Nature 290:221-225
• 23 Strains Carrying This Mutation

  Name	Mutations	Genotype
  G11h5	4	Hfr(PO2A), relA1, ilv-299, metB1, ampCp-1
  G11a1	5	Hfr(PO2A), relA1, spoT1, ilv-299, metB1, ampCp-1
  G11el	5	Hfr(PO2A), tolD4, relA1, ilv-299, metB1, ampCp-1
  D21	7	F-, proA23, lac-28, tsx-81, trp-30, his-51, rpsL173(strR), ampCp-1
  D21e19	8	F-, proA23, lac-28, tsx-81, trp-30, his-51, rpsL173(strR), rfa-11, ampCp-1
  D21e7	8	F-, proA23, lac-28, tsx-81, trp-30, his-51, rpsL173(strR), rfa-1, ampCp-1
  D31	8	F-, proA23, lac-28, tsx-81, trp-30, his-51, rpsL174(strR), rfa-229, ampCp-1
  TP1	8	F-, proA23, lac-28, tsx-81, trp-30, his-51, rpsL173(strR), ampCp-1, ampC11
  AKK211	9	Hfr(PO2A), λ-, relA1, spoT1, ilv-299, metB1, zjd-2211::Tn10, ampCp-1, mcrB9999, creC510
  AKK221	9	Hfr(PO2A), λ-, relA1, spoT1, ilv-299, metB1, zjc-2221::Tn10, ampCp-1, mcrB9999, creC510
  AKK231	9	Hfr(PO2A), λ-, relA1, spoT1, ilv-299, metB1, ampCp-1, cadB2231::Tn10, mcrB9999, creC510
  AKK241	9	Hfr(PO2A), λ-, relA1, spoT1, ilv-299, metB1, ampCp-1, zje-2241::Tn10, mcrB9999, creC510
  D21f1	9	F-, proA23, lac-28, tsx-81, trp-30, his-51, rpsL173(strR), rfa-21, rfa-1, ampCp-1
  D21f2	9	F-, proA23, lac-28, tsx-81, trp-30, his-51, rpsL173(strR), rfa-31, rfa-1, ampCp-1
  D22	9	F-, lpxC101, proA23, lac-28, tsx-81, trp-30, his-51, tufA1, rpsL173(strR), ampCp-1
  D23	9	F-, proA23, lac-28, tsx-81, trp-30, his-51, mreB11, rpsL173(strR), sloB1, ampCp-1
  D31m3	9	F-, proA23, lac-28, tsx-81, galU50, trp-30, his-51, rpsL174(strR), rfa-229, ampCp-1
  D31m4	9	F-, proA23, lac-28, tsx-81, trp-30, his-51, rpsL174(strR), rfa-229, rfa-230, ampCp-1
  TP3	9	F-, proA23, lac-28, tsx-81, trp-30, his-51, rpsL173(strR), ilv-631, ampCp-1, ampC3
  H1t3	14	F-, thr-20, leu-32, proA35, lacY1, tsx-70, glnX44(AS)?, gal-6, λ-, rpsL125(strR), malT1(λR), xyl-7, mtlA2, ampCp-1, purA45
  H1t14	15	F-, thr-20, leu-32, proA35, lacY1, tsx-70, glnX44(AS)?, gal-6, λ-, rpsL125(strR), malT1(λR), xyl-7, mtlA2, ampCp-1, ampC12, purA45
  HT306	16	F-, thr-1, araC14, ΔspeD98, Δ(gpt-proA)62, lacY1, glnX44(AS), galK2(Oc), λ-, Δ(speB-speA)97, Δ(speC-glcB)63, rpsL25(strR), xylA5, mtl-1, thiE1, ampCp-1, cadA2
  KL355	16	F-, fhuA2::IS2?, proA44, lacY1, glnX44(AS)?, galK2(Oc), λ-, hisG1, rfbC1, galP63, malT1(λR), xyl-7, mtlA2, ΔargH1, rplL9(L?), ampCp-1, purA44