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Mutation lacZ125(Am)

• Name: lacZ125(Am)
• Type: Base Pair Substitution
• Mutation of Gene: lacZ
• Approx. Map Location: 7.81
• Synonyms

  Designation	Where Used
  lac-Y125am	Brenner

  
  
• Properties

  Property	Symbol
  amber(UAG) mutation	Am

  
  
• Comments
  • is the amber mutation from Zipser's strain NG125 and Brenner's CA274. It was formerly called lac_125am by Brenner. In `interval 6' of Newton et al. JMB 14:290 (1965).
• References
  • Newton, W.A., J.R. Beckwith, D. Zipser, S. Brenner 1965. Nonsense mutants and polarity in the lac operon of Escherichia coli. J.Mol.Biol. 14:290-296
• 17 Strains Carrying This Mutation

  Name	Mutations	Genotype
  CA244	5	Hfr(PO1), lacZ125(Am), e14-, trpA49(Am), relA1, spoT1
  CA265	5	Hfr(PO1), lacZ125(Am), e14-, tyrT66(AS), trpA49(Am), relA1
  M7052	5	F-, lacZ125(Am), λ-, relA1, rpsL150(strR), spoT1
  CA266	6	Hfr(PO1), lacZ125(Am), e14-, trpA49(Am), serU67(AS), relA1, spoT1
  CA267	6	Hfr(PO1), lacZ125(Am), e14-, trpA49(Am), serU68(AS), relA1, spoT1
  CA273	6	Hfr(PO1), lacZ125(Am), lysT76(OS), e14-, trpA49(Am), relA1, spoT1
  CA274	6	Hfr(PO1), lacZ125(Am), λ-, e14-, trpA49(Am), relA1, spoT1
  K529	6	F-, lacZ125(Am), λ-, e14-, trpA49(Am), relA1, spoT1, pLM2
  CA275	7	Hfr(PO1), lacZ125(Am), λ-, e14-, tyrT66(AS), trpA49(Am), relA1, spoT1
  CA374	7	Hfr(PO1), lacZ125(Am), λ-, e14-, trpA49(Am), serU67(AS), relA1, spoT1
  JW1071	7	Hfr(PO1), lacZ125(Am), asnB507::Tn10, λ-, e14-, trpA49(Am), relA1, spoT1
  KLC1284	7	Hfr(PO1), lacZ125(Am), λ-, e14-, tyrT66(AS), trpC83::Tn10, relA1, spoT1
  CS130	11	F-, thr-1, araC14, leuB6(Am), lacZ125(Am), hisC3, metG155, metG146?, relA1, argA52, rpsL8 or rpsL25(strR), thiE1
  D-2	15	F-, ftsA2(ts), lacZ125(Am), tsx-465(Am), galK42(Am), galE54(GalS), λ-, tyrT77(AS), tyrT181(ts,AS), trpA49(Am), his-224, relA1, thyA756, spoT1, ilv-693, deo-84
  D-3	15	F-, ftsA3(ts), lacZ125(Am), tsx-465(Am), galK42(Am), galE54(GalS), λ-, tyrT77(AS), tyrT181(ts,AS), trpA49(Am), his-224, relA1, thyA756, spoT1, ilv-693, deo-84
  OV2	16	F-, araBAD-38(Am), leu-87, lacZ125(Am), tsx-465(Am), galK42(Am), galE54(GalS), λ-, tyrT77(AS), tyrT181(ts,AS), trpA49(Am), his-224, relA1, thyA756, spoT1, ilv-693, deo-84
  OV16	17	F-, araBAD-38(Am), leu-87, ftsA16(Am), lacZ125(Am), tsx-465(Am), galK42(Am), galE54(GalS), λ-, tyrT77(AS), tyrT181(ts,AS), trpA49(Am), his-224, relA1, thyA756, spoT1, deo-84, ilv-693