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Mutation DE(gltB-gltF)500

• Name: DE(gltB-gltF)500
• Type: Deletion
• Approx. Map Location: 72.25
• Synonyms

  Designation	Where Used
  aspB21	Paris, Lavalle
  aspB24	Matney, Baron, Glansdorff
  DE(gltB-gltD-gltF)
  gltB31

  
  
• Comments
  • :Glt^- phenotype in gltB mutant requires presence of a gdhA mutation as well. 0.2% aspartate is a better N-source than glutamate because of uptake(Pahel et al).
• References
  • Pahel, G, AD Zelenetz, BM Tyler 1978. gltB gene and regulation of nitrogen metabolism by glutamine synthetase in Escherichia coli. J. Bacteriol. 133(1):139-48.
  • Castano, I., F. Bastarrachea, A. Covarrubias 1988. gltBDF operon of Escherichia coli. J.Bacteriol. 170:821-827
• 16 Strains Carrying This Mutation

  Name	Mutations	Genotype
  BD2314	18	F-, fhuA2::IS2?, lacY1, tsx-70?, glnX44(AS)?, gal-6, λ-, trpC45, his-68, purC50, ung-152::Tn10, tyrA2, galP63, Δ(gltB-gltF)500, rpsL125(strR), malT1(λR), xyl-7, mtlA2, thiE1
  H865	18	F-, fhuA2::IS2, lacY1, tsx-70, glnX44(AS), gal-6, λ-, e14-, hns-1, trpC45, his-68, rfbC1, tyrA2, Δ(gltB-gltF)500, rpsL125(strR), malT1(λR), xyl-7, mtlA2, thiE1
  PA309	18	F-, thr-1, leuB6(Am), fhuA2::IS2, lacY1, glnX44(AS), gal-6, λ-, trp-1, hisG1, rfbC1, galP63, Δ(gltB-gltF)500, malT1(λR), xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1
  PA340	18	F-, thr-1, leuB6(Am), fhuA2::IS2, lacY1, glnX44(AS), gal-6, λ-, gdhA1, hisG1, rfbC1, galP63, Δ(gltB-gltF)500, malT1(λR), xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1
  H677	19	F-, fhuA2::IS2, lacY1, tsx-70, glnX44(AS)?, gal-6, λ-, e14-, hns-1, trpC45, his-68, purC50, tyrA2, galP63, Δ(gltB-gltF)500, rpsL125(strR), malT1(λR), xyl-7, mtlA2, thiE1
  JM73	19	F-, thr-1, leuB6(Am), fhuA2::IS2, lacY1, glnX44(AS), gal-6, λ-, trp-1, hisG1, rfbC1, cysJ90, galP63, Δ(gltB-gltF)500, malT1(λR), xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1
  JM96	19	F-, thr-1, leuB6(Am), fhuA2::IS2, lacY1, glnX44(AS), gal-6, λ-, trp-1, hisG1, rfbC1, cysH56, galP63, Δ(gltB-gltF)500, malT1(λR), xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1
  PA340/T6	19	F-, thr-1, leuB6(Am), fhuA2::IS2, lacY1, tsx-68, glnX44(AS), gal-6, λ-, gdhA1, hisG1, rfbC1, galP63, Δ(gltB-gltF)500, malT1(λR), xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1
  PC0177	19	F-, fhuA2::IS2, lacY1, tsx-70, glnX44(AS), gal-6, λ-, e14-, hns-1, trpC45, his-68, rfbC1, guaB27, tyrA2, Δ(gltB-gltF)500, rpsL125(strR), malT1(λR), xyl-7, mtlA2, thiE1
  H724	20	F-, fhuA2::IS2, lacY1, tsx-70, glnX44(AS)?, gal-6, λ-, e14-, hns-1, trpC45, his-68, rfbC1, purC50, guaB24, tyrA2, Δ(gltB-gltF)500, rpsL125(strR), malT1(λR), xyl-7, mtlA2, thiE1
  LCB358	20	F-, thr-1, leuB6(Am), fhuA2::IS2, pro-33, lacY1, purE43, glnX44(AS), gal-6, λ-, moaA15, hisG1, rfbC1, galP63, Δ(gltB-gltF)500, malT1(λR), xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1
  LCB382	20	F-, thr-1, leuB6(Am), fhuA2::IS2, pro-33, lacY1, purE43, glnX44(AS), gal-6, λ-, moaA1, hisG1, rfbC1, galP63, Δ(gltB-gltF)500, malT1(λR), xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1
  LCB442	20	F-, thr-1, leuB6(Am), fhuA2::IS2, pro-33, lacY1, purE43, glnX44(AS), gal-6, λ-, hisG1, rfbC1, galP63, Δ(gltB-gltF)500, malT1(λR), xyl-7, mtlA2, mobA2, ΔargH1, rplL9(L?), thiE1
  LCB498	20	F-, thr-1, leuB6(Am), fhuA2::IS2, pro-33, lacY1, purE43, glnX44(AS), gal-6, λ-, hisG1, rfbC1, galP63, Δ(gltB-gltF)500, malT1(λR), glgA1, xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1
  AA100	21	F-, thr-1, leuB6(Am), fhuA2::IS2, lacY1, glnX44(AS), gal-6, λ-, hns-1, trp-1, hisG1, rfbC1, galP63, Δ(gltB-gltF)500, fic-1, malT1(λR), xyl-7, mtlA2, rbsK3, ΔargH1, rplL9(L?), thiE1
  LCB618	22	F-, thr-1, leuB6(Am), fhuA2::IS2, pro-33, lacY1, purE43, glnX44(AS), gal-6, λ-, hns-1, hisG1, rfbC1, galP63, Δ(gltB-gltF)500, malT1(λR), glgC16?, glgC3, xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1