E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation trxA1(Am)

• Name: trxA1(Am)
• Type: Base Pair Substitution
• Mutation of Gene: trxA
• Approx. Map Location: 85.43
• Properties

  Property	Symbol
  amber(UAG) mutation	Am

  
  
• Comments
  • Gln98 to Amber codon. Growth of WT phage f1 in this strain is TS on all suppressors, whereas T7 grows at 42C.
• References
  • Mark, D.F., J.W. Chang, C.F. Richardson 1977. Genetic mapping of trxA, a gene affecting thioredoxin in Escherichia coli K12. Mol.Gen.Genet. 155:145-152
  • Russel, M., P. Model 1985. Thioredoxin is required for filamentous phage assembly. Proc.Natl.Acad.Sci.USA 82:29-33
• 2 Strains Carrying This Mutation

  Name	Mutations	Genotype
  DL16	11	F-, leu-64, lacZ53(Am), λ-, recA1, rpsL151(strR), ilvE720::Tn5, trxA1(Am), metE70, rha-5, malB45, deoC2
  A218	14	F-, garB10, fhuA22, phoA4(Am), ompF627(T2R), fadL701(T2R), relA1, pitA10, spoT1, ilvY864::Tn10, trxA1(Am), zif-2223::Tn5, rrnB-2, mcrB1, creC510