E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation glpD3

• Name: glpD3
• Type: Unknown
• Mutation of Gene: glpD
• Approx. Map Location: 76.73
• Comments
  • GlpD loss also results in stasis with gluconeogenic C sources, e.g.,succinate. glpA or glpD mutants should be grown on min.salts+1% glycerol-free casein hydrolysate+1% glucose.
  • Loss of G-3-P dehydrogenase (aerobic) results in inability of strains to grow on glycerol or glycerol-3-P as sole carbon source.
• References
• 17 Strains Carrying This Mutation

  Name	Mutations	Genotype
  BB20	12	Hfr(PO2A), fhuA22, ΔphoA8, fadL701(T2R), relA1, glpR2(glpc), glpD3, pitA10, gpsA20, spoT1, rrnB-2, mcrB1, creC510
  CV2-4	12	Hfr(PO2A), fhuA22, ΔphoA8, ushA1, ompF627(T2R), fadL701(T2R), glpR2(glpc), glpD3, pitA10, spoT1, rrnB-2?, mcrB1, creC510
  BB13	13	Hfr(PO2A), fhuA22, ΔphoA8, ompF627(T2R), fadL701(T2R), relA1, glpR2(glpc), glpD3, pitA10, spoT1, rrnB-2, plsB13, mcrB1, creC510
  BB20-14	13	Hfr(PO2A), fhuA22, ΔphoA8, fadL701(T2R), relA1, glpR2(glpc), glpD3, pitA10, gpsA20, spoT1, glpK15(fbR), rrnB-2, mcrB1, creC510
  CV15	13	Hfr(PO2A), fhuA22, ΔphoA8, adk-15(ts), ompF627(T2R), fadL701(T2R), relA1, glpR2(glpc), glpD3, pitA10, spoT1, rrnB-2, mcrB1, creC510
  CV2	13	Hfr(PO2A), fhuA22, ΔphoA8, adk-2(ts), ompF627(T2R), fadL701(T2R), relA1, glpR2(glpc), glpD3, pitA10, spoT1, rrnB-2, mcrB1, creC510
  CV31	13	Hfr(PO2A), fhuA22, ΔphoA8, adk-31(ts), ompF627(T2R), fadL701(T2R), relA1, glpR2(glpc), glpD3, pitA10, spoT1, rrnB-2, mcrB1, creC510
  Lin 8	13	Hfr(PO2A), garB10, fhuA22, ΔphoA8, ompF627(T2R), fadL701(T2R), relA1, glpR2(glpc), glpD3, pitA10, spoT1, rrnB-2, mcrB1, creC510
  Lin 9	13	Hfr(PO2A), fhuA22, ΔphoA8, ompF627(T2R), fadL701(T2R), relA1, glpR2(glpc), glpD3, pitA10, spoT1, glpK18, rrnB-2, mcrB1, creC510
  10B5	14	Hfr(PO2A), garB10, fhuA22, ΔphoA8, ompF627(T2R), fadL701(T2R), relA1, glpR2(glpc), glpD3, pitA10, spoT1, pst-2, rrnB-2, mcrB1, creC510
  Lin 135	14	F-, thr-1, leuB6(Am), secA208, fhuA2::IS2, lacY1, glnX44(AS)?, sdh-3, λ-, rpsL135(strR), glpR2(glpc), glpD3, xyl-7, mtlA2, thiE1
  SH210	14	Hfr(PO2A), garB10, fhuA22, Δ(argF-lac)169, zai-736::Tn10, ΔphoA8, ompF627(T2R), fadL701(T2R), relA1, glpR2(glpc), glpD3, pitA10, spoT1, mcrB9999, creC510
  BB26-36	15	Hfr(PO2A), fhuA22, ΔphoA8, ompF627(T2R), plsX50, fadL701(T2R), relA1, glpR2(glpc), glpD3, pitA10, spoT1, glpK14(fbR), rrnB-2, plsB26, mcrB1, creC510
  GN1908	15	F-, ΔphoA8, galK35, λ-, pyrD34, his-68, thyA25, rpsL118(strR), glpR2(glpc), glpD3, pitA10?, xyl-7, mtlA2, spoT1?, glpK4, thiE1
  PLS73	16	F-, ΔphoA8, galK35, λ-, pyrD34, his-68, fadL701(T2R), thyA25, rpsL118(strR), glpD3, pitA10, xyl-7, mtlA2, spoT1, glpK4, thiE1, pls-73
  GN3	17	F-, ΔphoA8, adk-3(ts), galK35, λ-, pyrD34, his-68, fadL701(T2R), thyA25, rpsL118(strR), glpR2(glpc), glpD3, pitA10, xyl-7, mtlA2, spoT1, glpK4, thiE1
  GN9	17	F-, ΔphoA8, adk-9(ts), galK35, λ-, pyrD34, his-68, fadL701(T2R), thyA25, rpsL118(strR), glpR2(glpc), glpD3, pitA10, xyl-7, mtlA2, spoT1, glpK4, thiE1