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CGSC, The Coli Genetic Stock Center

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Mutation thyA25

• Name: thyA25
• Type: Unknown
• Mutation of Gene: thyA
• Approx. Map Location: 63.86
• Comments
  • Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• References
• 18 Strains Carrying This Mutation

  Name	Mutations	Genotype
  FP4102	8	F', galE66(GalS), λ-, trpA9761(Am), Δ(his-gnd)81, gyrA12(NalR), recA1, thyA25, rpsL229(strR), F410
  KL162	8	Hfr(PO45), λ-, e14-, recA1, relA1?, thyA25, spoT1?, thiE1, deoB13
  KL163	8	F-, λ-, e14-, gyrA12(NalR), recA1, relA1?, thyA25, thiE1, deoB13
  KL141	10	F-, λ-, relA1?, thyA25, argG6, gltB17, rpsL8 or rpsL104 or rpsL17, malT1(λR), pyrE41, rbs-1, thiE1
  GS5	12	F-, lacY1 or lacZ4 or lacY29, proC24, tsx-63?, purE41?, pyrF30, his-53, gyrA12(NalR), thyA25, rpsL97(strR), pitA10, pst-2, metB1
  KL190	12	F-, galK35, λ-, pyrD34, trpC45, his-68, thyA25, thyA33, rpsL118(strR), malT1(λR), xyl-7, mtlA2, thiE1
  KL188	14	F-, galK35, λ-, pyrD34, hns-1?, trpC45, his-68, rfbC1?, thyA25, galP63?, rpsL118(strR), malT1(λR), xyl-7, mtlA2, thiE1
  GN1908	15	F-, ΔphoA8, galK35, λ-, pyrD34, his-68, thyA25, rpsL118(strR), glpR2(glpc), glpD3, pitA10?, xyl-7, mtlA2, spoT1?, glpK4, thiE1
  KL733	15	F', thr-1, leuB6(Am), fhuA2::IS2, pro-27, lacY1, gal-6, λ-, hisG1, recA1, thyA25, malT1(λR), xyl-7, rplL9(L?), thiE1, pyrB31, F118
  KL734	15	F', thr-1, leuB6(Am), fhuA2::IS2, pro-27, lacY1, gal-6, λ-, hisG1, recA1, thyA25, malT1(λR), xyl-7, rplL9(L?), thiE1, pyrB31, F119
  KL735	15	F', thr-1, leuB6(Am), fhuA2::IS2, pro-27, lacY1, gal-6, λ-, hisG1, recA1, thyA25, malT1(λR), xyl-7, rplL9(L?), thiE1, pyrB31, F121
  KL132	16	F-, thr-1, leuB6(Am), fhuA2::IS2, pro-27, lacY1, gal-6, λ-, hisG1, recA1, relA1?, thyA25, malT1(λR), xyl-7, rplL9(L?), thiE1, pyrB31
  PLS73	16	F-, ΔphoA8, galK35, λ-, pyrD34, his-68, fadL701(T2R), thyA25, rpsL118(strR), glpD3, pitA10, xyl-7, mtlA2, spoT1, glpK4, thiE1, pls-73
  GN3	17	F-, ΔphoA8, adk-3(ts), galK35, λ-, pyrD34, his-68, fadL701(T2R), thyA25, rpsL118(strR), glpR2(glpc), glpD3, pitA10, xyl-7, mtlA2, spoT1, glpK4, thiE1
  GN9	17	F-, ΔphoA8, adk-9(ts), galK35, λ-, pyrD34, his-68, fadL701(T2R), thyA25, rpsL118(strR), glpR2(glpc), glpD3, pitA10, xyl-7, mtlA2, spoT1, glpK4, thiE1
  KL218	17	F-, lacY1 or lacZ4 or lacY29, proC24, tsx-63?, purE41, λ-, pyrF30, his-53, gyrA12(NalR), thyA25, argG34 or argG35, rpsL97(strR), xyl-7 or xyl-14, mtlA2, metB65?, metB1, cycA1?, cycB2?
  KL732	17	F', thr-1, leuB6(Am), fhuA2::IS2, pro-27, lacY1, glnX44(AS)?, gal-6, λ-, hisG1, recA1, thyA25, malT1(λR), xyl-7, mtlA2?, rplL9(L?), thiE1, pyrB31, F117
  KL219	18	F-, lacY1 or lacZ4 or lacY29, proC24, tsx-63?, purE41, λ-, pyrF30, his-53, gyrA12(NalR), recA1, thyA25, argG34 or argG35, rpsL97(strR), xyl-7 or xyl-14, mtlA2, metB1, cycA1?, cycB2?, met-65?