E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation trpC83::Tn10

• Name: trpC83::Tn10
• Type: Tn
• Mutation of Gene: trpC
• Approx. Map Location: 28.38
• Synonyms

  Designation	Where Used
  trpB83::Tn10

  
  
• Properties

  Property	Symbol
  tetracycline resistant

  
  
• Comments
  • PCR analysis by Nichols et al. 1998 placed this insertion in the trpC rather than trpB gene (at nucleotide 1,317,596 or 28.401 min.)
• References
  • Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.
• 5 Strains Carrying This Mutation

  Name	Mutations	Genotype
  CAG18455	3	F-, λ-, trpC83::Tn10, rph-1
  NK7402	3	F-, λ-, trpC83::Tn10, IN(rrnD-rrnE)1
  CAG5054	5	Hfr(PO44), λ-, trpC83::Tn10, relA1, spoT1, thiE1
  DL39W	6	F-, λ-, aspC13, trpC83::Tn10, fnr-25, ilvE12, tyrB507
  KLC1284	7	Hfr(PO1), lacZ125(Am), λ-, e14-, tyrT66(AS), trpC83::Tn10, relA1, spoT1